利血平的光化学荧光特性及其应用

在10％HAc介质中,利血平经紫外光照射时,其光化学反应产物具有强烈荧光(λ_(ax)=385 nm,λ_(ax)=490 nm)。利血平浓度在0.01～0.30μg/ml范围内,荧光强度与浓度呈良好的线性关系,由此建立了利血平的光化学荧光测定法。方法的检出限为1.3 ng/ml,相对标准偏差为1.6％。本法简便快速且灵敏度高,可用于片剂及注射液中利血平含量的测定。     

C反应蛋白免疫电极的研究

C反应蛋白(CRP)是临床医学中重要的检验项目之一。本文以醋酸纤维素-戊二醛-己二胺载体膜与自制的原电极研制成非标记CRP免疫电极。研究了载体膜的组成对电极灵敏度的影响,并采用均匀设计法对载体膜的活化条件、抗原抗体结合时间以及电极测试条件等进行了优化。该电极响应快、灵敏度高、重现性和稳定性良好。在最佳实验条件下,电极线性范围为1.8～60μg/ml,所得线性回归方程为E=0.2+0.091[CRP];相关系数0.9989。在用于血清样品的测定中,取得满意结果。     

Green-synthesized,low-cost tetracyanodiazafluorene (TCAF) as electron injection material for organic light-emitting diodes

As facile,green,low-cost as possible:One more electron-deficient azaacene (TCAF) with deep LUMO (-4.52 eV), strong electronic affinity,excellent yield,and simple purification procedure was successfully created and explored as good electron injection material.It is believed TCAF would be a promising and pervasive acceptor material and bring in more significant achievements to green and sustainable organic electronics including OLEDs,OFETs,OPVs, and perovskite solar cells,etc.     

Nitrile biotransformations for highly efficient and enantioselective syntheses of electrophilic oxiranecarboxamides

Catalyzed by a nitrile hydratase/amidase-containing microbial Rhodococcus sp. AJ270 whole-cell catalyst, a number of racemic trans-2,3-epoxy-3-arylpropanenitriles 1 underwent rapid and efficient hydrolysis under very mild conditions to afford 2R,3S-2-arylglycidamides 2 in excellent yield with enantiomeric excess higher than 99.5%. The overall enantioselectivity of the biotransformations originated from the combined effects of a dominantly high 2S-enantioselective amidase and low 2S-enantioselective nitrile hydratase involved in the cell. The influence of the substrates on both reaction efficiency and enantioselectivity was also discussed in terms of steric and electronic effects.     

2-氨基嘧啶对铜的缓蚀机理

利用动电位极化曲线、线性极化、X射线光电子能谱(XPS)、X射线俄歇能谱(X-AES)和表面增强拉曼散射(SERS)研究2-氨基嘧啶(2-AP)对金属铜的缓蚀作用。结果表明: 在含Cl~-离子的酸性介质中, 2-AP是铜的有效缓蚀剂; 随着介质pH值的增加, 2-AP的缓蚀能力降低。2-AP缓蚀能力随pH值明显变化的原因在于只有在酸性介质中, 质子化了的2-AP分子、Cl~-离子和铜表面原子(或离子)才可能形成保护作用强的多聚配合物膜。     

毛细管气相色谱法同时测定水产品中的多氯联苯和有机氯农药残留量

建立了毛细管气相色谱法同时测定水产品中多氯联苯和有机氯农药的方法，用高氯酸与冰乙酸的混合溶液消化样品，以石油醚提取，浓硫酸净化后，用毛细管气相色谱法测定，方法的回收法为88％－110％，相对标准偏差为8％－19％（n=6)，检出限为0．08－0．60ug/kg，方法简便，快速，结果准确。     

气相色谱-质谱法分析木奶果挥发油的化学成分

采用水蒸汽蒸馏法从大戟科木奶果中提取挥发油,用气相色谱-质谱对挥发油的化学成分进行了分析。鉴定出19种化合物,占总油量的100%,用面积归一化法测定了各成分的相对百分含量。木奶果挥发油主要成分为正十六碳酸、丁基甲氧基苯、9,12-十八碳二烯酸,分别占挥发油总量的29.53%、17.47%和11.59%,三者占其总量的58.59%。     

Synthesis and Crystal Structure of 1，5－Bis（1＇－phenyl－3＇－methyl－5＇－pyrazolone－4＇）－1－（4＇－aminobutand－1＇－imino）－5－pentaneone

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Affinity NMR: decoding DNA binding

We have shown that affinity NMR can be used to edit a NMR spectrum so that ligands that have affinity to DNA can be observed in the presence of other nonbinding molecules. Diffusion encoded spectroscopy (DECODES) can be used to identify the binding ligands. We were able to identify Hoechst 33342 as binding to the Drew-Dickerson dodecamer d(CGCGAATTCGCG)2 in the presence of the nonbinding molecules adenine, adenosine, and thiamine. Affinity NMR appears to be readily applicable to DNA systems for the following reasons. (1) The relaxation rate of the DNA oligonucleotides is favorable, thus the signal intensity loss due to relaxation is not severe. (2) A comparison of the patterns of the DNA cross-peaks upon binding in the two-dimensional total correlation spectroscopy and correlation spectroscopy spectrum are easily performed, and the ligand signals in the two-dimensional DECODES spectrum can be readily identified. (3) The aromatic part of the DNA spectrum is devoid of 2D cross-peaks in these correlation spectra, greatly facilitating the interpretation of the bound ligand in the DECODES spectrum.     

Surface nuclear spin relaxation of 199Hg

We report the results of a detailed study of surface relaxation of 199Hg nuclear spins in paraffin coated cells. From measurements of the magnetic field and temperature dependence of the spin relaxation rates we determine the correlation time for magnetic fluctuations and the surface adsorption energy. The data indicate that surface relaxation is caused by dipolar coupling to paramagnetic sites on the surface. We also observe changes in the spin relaxation rate caused by ultraviolet radiation resonant with the 254 nm transition in Hg.